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Reactions were incubated at 30 C for 1 h and then terminated by the addition of sodium dodecyl sulfate (SDS) sample buffer

On the other hand, pro-apoptotic miRNAs, like the let-7 and miR-15 families, are downregulated, thus inhibiting apoptosis and promoting tumor progression (Mahboobnia et al

The compounds were carried out for xanthine oxidase inhibition activity, and both 21 , 22 were reported to exhibit xanthine oxidase inhibition [55] another 12 compounds were isolated from the same species, already known compounds 1 , 4 , and 8 , along with glucosyringic acid (25 ),benzyl-O--D-glucopyranoside (26 ), p-Hydroxycinnamic-acid ( 27 ) and 3,5-dihydroxy-4-Methoxybenzoic acid (28 ).These compounds were reported to be identified by comparing their 1H and 13C NMR data to spectroscopic data available in the literature [56]

Animals were housed in pathogen-free animal holding rooms, and experimental and control groups were co-housed