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Deletion of the key catalytic enzyme methylthioadenosine phosphorylase (MTAP) gene is associated with the sensitivity of GSK3368715, 331 and a current phase II clinical study of GSK3368715 in breast cancer has been enrolled (NCT04676516) (Table 1)

Indeed, as soon as it hatches (23 dpf), the zebrafish embryos start to feed on prey, which requires the maturation of its gastrointestinal apparatus, the inflation of its swim bladder, and the maturation of its craniofacial cartilages

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GST-tag from the target protein is often necessary due to its large size