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This formula has a history of over 2,000 years in China and is widely employed to treat various types of depression due to its liver-soothing, depression-relieving, spleen-nourishing, and blood-nourishing effects (Li et al., 2022)

139 , 487492 (1984) Lichtenthaler, H.K.: Chlorophylls and carotenoids: pigments of photosynthetic biomembranes

After centrifugation, 800 L 2.5 mol L 1 H 2 SO 4 was used to fully dissolve the precipitate, followed by centrifugation at 8,000 g for 5 min at 20 C

Notably, Ataluren forms hydrogen bonds with His 153 and Arg 174 , a salt bridge with Arg 174 , and a -stacking interaction with Phe 113