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Annu Rev Immunol 27:147163 Del Campo JA, Gallego P, Grande L (2018Jan 27) Role of inflammatory response in liver diseases: therapeutic strategies

10.1128/CMR.00081-09 [DOI] [PMC free article] [PubMed] [Google Scholar] Day C

3 Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia

Samples were resuspended in 160 L EPPS buffer (200 mM, pH 8.0) using a probe sonicator (1 1015 pulses, power output 20%) and then digested with LysC (4 L of 0.5 g/L per sample, resuspended in HPLC grade water, Wako-chemicals, Fujifilm #125-05061) for 2 h at 37 C in a shaker incubator