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For any synthetic AT developed, it is important to know which biomolecule the agent is designed to protect, by which mechanism (scavenging RS, preventing RS formation, increasing endogenous defense mechanisms, and/or supporting oxidative damage repair), whether it can generate RS, and if there are any adverse effects associated with RS suppression

The slides were incubated with primary antibodies in a Tris-buffered saline, 0.1% Tween 20 Detergent solution (TBST) overnight in a humidified chamber at 4C