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For tissue, the protocol requires liquid nitrogen freezing within 5 seconds of excision, pulverization in a liquid-N-cooled mortar, homogenization in ice-cold methanol containing 0.1% BHT (antioxidant) and 10 M indomethacin, centrifugation, dilution to 5:1 in plasma suggests ex vivo leukotriene generation because LTA4 generated ex vivo by activated neutrophils can non-enzymatically conjugate with glutathione in red blood cells

Without published stability data for nasal formulations, users should treat opened solutions as unstable at room temperature

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