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Samples were separated on a 12% SDS-PAGE gel and transferred onto a nitrocellulose membrane

Stable-isotope-labeled internal standards can help correct extraction loss, matrix effects, and instrument variability

Model analysis Selection of parameters for estimation The methodology to select parameters for estimation involves first conducting local sensitivity analysis (LSA) to obtain a set of important parameters, and then determining from this set of parameters which are identifiable, that is for which parameters a value can be identified from the data, taking into account the model structure

When methionine flux is directed toward glutathione synthesis, the cycle aids in cellular defense against oxidative stress