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After centrifugation, the supernatant solution was collected and purified using semi-preparative HPLC, C4 column and gradient of 2060% B to give the desired product in ~45% yield (Supplementary Fig

After tandem purification using GST and strep affinity tags, we obtained the protein sample for cryo-EM data collection (Fig

Nonetheless, certain individuals might encounter mild adverse effects including nausea, bloating, and certain dermal issues like itching and increased perspiration