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The cell precipitate was resuspended in Neurobasal medium (Gibco, 21103049) supplemented with 2% B-27 (Gibco, 17504044) and 0.5 mM l-glutamine, and the cells were seeded on plates pre-coated with poly-d-lysine hydrobromide (Sigma-Aldrich, P0296)

In a 2001 study published in Endocrinology , Heffernan and colleagues reported that chronic AOD-9604 administration reduced body weight gain, decreased adipose tissue mass, and increased fat oxidation in obese mice without altering IGF-1 levels or glucose metabolism effects that were abolished in beta3 adrenergic receptor (3-AR) knockout mice, implicating 3-AR signaling as the mechanism of action [1]