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Tissue samples were sonicated in PBS supplemented with protease and phosphatase inhibitors (Complete protease inhibitor cocktail, Roche)

10.13376/j.cbls/20220077 67 LiJ

To understand the role of ROS in the regulation of EC proliferation by exogenous GSH and ROS metabolism response pattern, this study increases the GSH content of Korean pine EC cells by adding exogenous GSH while decreases it by adding exogenous l-Buthionine-sulfoximine (BSO) to investigate the dynamic change of the effects of exogenous GSH and BSO on nitric oxide (NO), hydrogen peroxide (H 2 O 2 ), catalase (CAT), superoxide dismutase (SOD), cell death, cell division ratio, and malondialdehyde (MDA) in Korean pine EC cells, and to reveal the change pattern of ROS metabolism in the proliferation of Korean pine EC cells promoted by exogenous GSH