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Description

126 , e83 (2019)

Espinosa-Diez C, Miguel V, Mennerich D, Kietzmann T, Snchez-Prez P, Cadenas S, et al

To remove the GST tag, PreScission Protease (95017-584, VWR) was added following the manufacturers directions, and the protein/enzyme mixture was dialyzed against GST wash buffer (50 mM Tris, pH 8, 400 mM NaCl, 1 mM EDTA, 3 mM DTT) at 4 C over night