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Disease-anchored panels and how to act on them For disease-anchored panels, tailor the readouts to the biology and act on the map they provide: in cardiovascular/vascular beds, pair DHE-HPLC (to quantify O 2 ) with compartment-targeted HyPer for H 2 O 2 , then add F 2 -isoprostanes/3-nitrotyrosine and iron indices to distinguish NOX-driven endothelial dysfunction (O 2 ONOO lowering NO bioavailability) from mitochondrial bursts typical of ischemiareperfusion (Bkgaard Nielsen et al

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The concentration of Human GDNF in the samples is then determined by comparing the OD of the samples to the standard curve

Zebrafish: an emerging model system for human disease and drug discovery