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An additional set of aromatic amides with unknown in vivo metabolism were selected using a systematic approach to investigate substituent effects of e.g., electron withdrawing (-NO 2 , -F) and electron donating groups (-OH, -CH 3 ) which were varied between compounds around the formanilide ring ( ortho -, meta - and para - to the amide bond) for future quantitative structure-activity relationship (QSAR) modelling

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During hypoxia or mitochondrial dysfunction, KG can be converted to citrate in an IDH2-catalyzed reductive carboxylation reaction