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This creates a vicious cycle, where impaired waste clearance contributes to ROS buildup, which in turn promotes neuroinflammation, alters sleep architecture, and leads to further fragmentation of sleep (Gu et al., 2022

this actually started when I was 47, but I procrastinated for 2 years before I decided to try TRT

It has been reported that GFP fluorescence can respond to subtle environmental differences caused by POI unfolding (before aggregation), and the resulted changes in intensity (decrease in those cases) can be used directly to monitor unfolding by differential scanning fluorimetry 80

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