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The compound additionally helps in protecting cells from oxidative damage

Beads were washed four times with IP wash buffer (50 mM TrisHCl (pH 7.4), 150 mM NaCl, 0.1% (vol/vol) IGEPAL CA-630), then thrice with IP wash buffer lacking IGEPAL CA-630

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It can be synthesized using different petroleum-based chemical methods, including (i) nitric acid oxidation of 2-cyanocylopentanone, (ii) condensation of acrylonitrile with ethyl malonate, (iii) ozonation of cyclopentane and permanganate cleavage of ozonide, and (iv) oxidation of pentamethylene glycol using nitrogen tetroxide 3,4,5