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To identify mechanisms directly affected by Gsta4/4-HNE, we analyzed RNA-seq data of corpus callosum-derived O4 + OLs to identify crucial molecules involved in these processes, using the following criteria: (1) significant differential expression ( P 0.001) between DA Wt and DA Gsta4 (Supplementary Data 2)

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Authors contributions DCO participated in the design of the study and wrote the introduction and molecular mechanism and gene expression sections

Other pathways that have been implicated in hepatocyte injury and the development of NASH, although rather broadly, include cholesterol accumulation in hepatocytes (96) and a tangled web involving activation of apoptotic pathways with ER stress and abnormal unfolded protein response (97), as well as defects in autophagy (98)