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Dairy cows genetically selected for high milk production are particularly susceptible to heat stress due to elevated ambient temperatures, which reduce feed intake while increasing metabolic heat production and overall heat load (Loor et al., 2023

doi: 10.1016/j.neuint.2012.11.017

After fixation with 1% osmium acid solution at room temperature for 1.5 h, the samples were washed three times with 0.1 M phosphate buffer and gradually dehydrated in ethanol (50-100%)