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The need to maintain a homeostatic relationship with the microbiota has been a driving factor force in the evolution of the human immune system and to keep the gut microbiota for its essential role in host metabolism and functions ( This alliance was reached by a fine-tuned contact modulation between gut bacteria and intestinal epithelial cells and by limiting the possible entrance of bacteria trough the mucosal layer

Slightly positive or near-neutral zeta potentials (approximately 10 to +10 mV) are generally preferred for systemic administration because highly cationic particles can strongly bind to the negatively charged endothelial glycocalyx and plasma proteins, causing aggregation, complement activation, and membrane damage (120)

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NS indicates not significant