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After 30 h, the cells that had migrated through the membrane of the insert were fixed with 4% paraformaldehyde and stained with 0.1% Crystal Violet

Given that both cysteine and GSH contribute to cell metabolism, which may be a confounding factor in MTT assay, we verified the extent of cell death by propidium iodide (PI) staining

Store at controlled room temperature away from direct sunlight and excessive heat

Applications in Research As a standard diluent for peptide and protein reconstitution Preparation of injectable-grade research compounds for in vitro or ex vivo studies Investigation of peptide and biologic stability following reconstitution Formulation testing and solvent compatibility studies Multi-use experimental workflows requiring bacteriostatic protection Specifications Summary Purity: Manufactured to specification Appearance: Clear, colourless sterile solution Solvent Type: Bacteriostatic aqueous diluent Preservative: Benzyl alcohol 0.9% (w/v) Solubility: Fully miscible with water and aqueous buffers Storage: Controlled room temperature Pack Size: 10 ml Handling, Use & Stability Use aseptic technique when accessing vial contents Avoid contact with non-sterile surfaces Do not use if solution becomes cloudy or discoloured Seal vial promptly after use to maintain sterility Avoid exposure to excessive heat or prolonged light Do not freeze Precautions & Notes Not all peptides or biologics are compatible with benzyl alcohol