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Next, denatured protein samples (15 g per lane, diluted in 5 loading buffer and boiled at 95 C for 5 min) were separated on 10% SDS-PAGE gels and transferred to PVDF membranes (Millipore, USA) using a Pyxis Gel Processor (China)

In LPS-treated mice, the expression of Muc-2 and Muc-4 was downregulated, which was reversed by supplement of Lactobacillus rhamnosus LAB3 and Lactobacillus plantarum LAB39, indicating the effect of the above two strains in protecting mucus barrier in LPS-induced colitis [43]

Further applications of the ketopinic acid framework as a chiral auxiliary relied on fine tuning of the steric environment around the carbonyl group

To investigate the function of CBS in this process, we silenced its expression by CRISPR/cas9 mediated gene knockout strategy (sg CBS ) in SCD erythroblasts (Fig