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Perez-Pardo et al (51) further explored the role of Toll-like receptor 4 (TLR4) in the gut-brain axis in PD, suggesting that TLR4 signaling may be a therapeutic target

Flow-cytometry analysis To isolate hepatic MNCs, the inferior vena cava was cut above the diaphragm and the liver was flushed with cold PBS until it became pale

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