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Elasticity modulus assay Lung tissue elasticity modulus were analyzed using the Mechanical Testing System (MACH-1 V500C, Biomomentum Inc., Laval, Canada)

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Blots were blocked with phosphate buffered saline with 0.1% Tween 20 and then binding with antibodies for eNOS (1: 1000) or caveolin-1 (1: 1000), followed by horseradish peroxidase-conjugated goat anti-rabbit (diluted 1: 5000) and enhanced chemiluminescence reaction was performed for blots development