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Under physiological conditions, a small proportion of electrons (approximately 2%) prematurely leak from the ETC prior to reaching complex IV, leading to the partial reduction of oxygen and the formation of superoxide [78]

The absorption at 420 nm and 550 nm of the supernatant was measured against a mixture of 100 L lysis buffer, 900 L resuspension buffer, 100 L 0.4 % pNPP solution and 200 L stop solution in a BioSpectrophotometer (Eppendorf, Hamburg, Germany)

How to use: Pump a small amount and spread evenly all over your face and neck

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