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Briefly, this vector was derived from a commercially available plasmid (VectorBuilder, Cat #VB010000-9782ymj) from which the f1 origin of replication and the lac operon system were removed to decrease the plasmid size

No differences in 5-HT2A receptors in mid-life depression or LLD, lower 5HT2A receptors in mid-life depression in orbital-frontal cortex, and higher 5-HT2A receptors have been reported [109,110,111,112]