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Furthermore, immunoblotting data revealed a decreased expression of CBS (Fig

doi: 10.1126/sciimmunol.abg7836 32 OMeliaMJManspeakerMPThomasSN

The protein was eluted in wash buffer with 250 mM imidazole and treated with Lamda Phosphatase (New England BioLab) and Alkaline Phosphatase (New England BioLab) overnight at 4 C