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Using FACS analysis and coupling labeled aggregate treatment with an Islet1-GFP motor neuron embryonic stem cell (ESC) reporter line 34 , we were able to determine the specific MN accumulation of DyLight 650 labeled SOD1 H46R aggregate uptake (GFP positive and DyLight 650 positive) and compare it to that of the Islet1 negative cells (GFP negative and DyLight 650 positive) within the same culture