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The cell pellet was resuspended in FxCycle PI/RNase Staining Solution (Invitrogen, Thermo Fisher Scientific, Inc) and the dye was incubated for 30 min at room temperature (RT)

Restoring EC function prevents cachexia To assess the therapeutic effects of inhibiting the activin APGC1 axis in the muscle endothelium, we first examined whether inhibition of activin A signaling using an anti-activin A neutralizing antibody could restore EC function and prevent muscle loss in cancer cachexia (Extended Data Fig