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Levels of AKT phosphorylation were detected by immunoblotting using the pS473-AKT antibody

Nature 423 , 550555 (2003)

The restoration of mTOR signaling with Fer1 rescue treatment in the context of RSL3 effects, and the genetic knockdown of GPX4, reaffirm our in vitro findings that RSL3-mediated induction of ferroptosis, mTOR pathway suppression, DNA damage and failure of DNA repair, and autophagy are GPX4-dependent processes in thyroid cancer cells