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In our initial attempts, we noticed substantial ER and mitochondrial contamination in the precipitates (Figure 2D, third lane)

The degenerative process of intervertebral discs is often accompanied by the overexpression of matrix metalloproteinases (MMP3, MMP13) and inflammatory factors (IL-1, IL-6, IL-8, PEG2, TNF-) (Wang et al., 2020)