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Reduced glutathione (GSH/GSSG) assay For the detection of reduced glutathione and GSSG levels in tumor tissues and cell lysate, the GSH Assay Kit (Sigma-Aldrich) and GSSG assay kit (Sigma-Aldrich) were used following the manufacturers instructions

It plays a vital role in protecting cells from damage caused by harmful free radicals and toxins, which can lead to a wide range of health problems

Our results showing that leucovorin increases oligodendrogenesis and (re)myelination both in vitro and in vivo suggest that it promotes these processes at least in part by fostering high levels of folate metabolism

However, the application of these drugs requires strict control of indications and contraindications to avoid unnecessary damage to patients