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Mechanisms for back pain: GHK-Cu stimulates collagen synthesis, supporting the structural proteins in discs, ligaments, and joint capsules

doi: 10.3390/nu7042485 70 KivitySAgmon-LevinNZisapplMShapiraYNagyEVDankKet al

Immunoprecipitation (IP) and Co-immunoprecipitation (Co-IP) assay Cells or tissues were lysed in IP lysis buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.5% Triton X-100, 0.5% NP-40, 1 mM EDTA, and 1 mM EGTA, 1 mM -glycerophosphate, 1 mM PMSF, and 1X PIC)

Despite the borderline significance observed between the groups during the 9-year period, it is important to interpret the results cautiously due to the lack of significant difference in the multivariable-adjusted Cox regression analysis