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Unlike PELD, PPECD has limited manipulation space, and once anesthesia-related complications, such as total spinal cord anesthesia [7], occur, the results will be disastrous

10.3389/fgene.2019.01299 147 WanY.McdanielK.WuN.Ramos-LorenzoS.GlaserT.VenterJ.et al (2017)

Cells were washed twice with warm PBS and fixed with the Karnovsky fixative as described above at room temperature for an hour

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