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After three PBS washes, iHLOs were incubated in 100 L Ce3D permeabilization/blocking buffer (BioLegend, catalog: 427706) for 1 h at room temperature

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Results in laboratory animals have shown that these irreparably damaged cells contribute to conditions such as cancer, loss of muscle mass and strength, liver damage, cardiovascular disease, and forms of neurodegeneration (diseases of the nervous system)

This is the first stage where a before-and-after comparison would show a noticeable difference