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Recently, the PC-synthesis capability and the presence of constitutive and functional PCS were considered as ancestral (plesiomorphic) characters for basal land plants including bryophytes, charophytes, and lycophytes (Petraglia et al., 2014)

The Real-Time PCR was performed with a CFX ConnectTM Real-Time PCR Detection System (BIO-RAD, Warsaw, Poland) and following cycling conditions: 95 C for 2 min, followed by 40 cycles at 95 C for 15 s, annealing for 15 s, and elongation at 72 C for 15 s