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After preheating at 37 C for 5 min of the reaction mixture (500 L) with 0.1 M NaCl (350 L) and plant extract (50 L), the enzyme reaction was initiated by adding 100 L of 100 U/ml ADH (Merck, Germany) for NAD(H) determination, or 100 L of 14 U/ml G6PDH (Merck, Germany) for NADP(H) determination