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Jiang, F.-Z

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To add additional landmarks, we generated the outline around the head using the publicly available program tpsDig2w64 (( 2.6 Geometric morphometrics and statistical analyses of facial images We imported the generated TPS files for each fetus into the MorphoJ software (( We then used Canonical Variate (CV) analysis to identify differences in facial features between treatments and exported the raw CV scores into the publicly available Paleontological Statistics Software Package for Education and Data Analysis (PAST) analysis software (( We conducted multivariate analyses of the raw CV scores using statistical methods described previously ( 2.7 Data handling and statistical analysis We subjected all data generated during this study to the data management practices and statistical analyses described previously ( We transferred the collected datasets into GraphPad Prism 10 (RRID:SCR_002798, GraphPad Software Inc., La Jolla, CA, United States)

Lastly, further longitudinal studies could be designed using different doses of NAC and evaluate the persistency of the effect of the medication after treatment to define the optimum dose(s) that can be translated into preclinical and clinical settings