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PubMed, Yen, Ju-Yu, et al

Simplistic conventional, also inappropriately called 2D, monolayer cultures with a single layer of cells attached to treated polystyrene have unnaturally low cell densities, lack cell-cell interaction or cell-peri and extracellular matrix interactions, and lack the exhibition of the brain tumor nutrient gradients or physiological levels of oxygen (112)

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Liposomal Vitamin C: Strengthening the Glutathione Network Liposomal Vitamin C: Strengthening the Glutathione Network Vitamin C and glutathione are biochemically interdependent