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SKU: 50488452981
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4-HNE protein adducts were detected by an anti 4-HNE protein adduct antibody (1:2000, AB5605, Millipore) in Tris-buffer saline with 5% skim milk, and ECL (GE Healthcare)

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Together, these findings demonstrate that ferroptosis is induced in experimental diabetic nephropathy through the effect of TGF-1 and that treatment with Fer-1, a ferroptosis inhibitor, attenuated kidney cell death in diabetic mice (Fig