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Mutagenesis Site-directed mutagenesis of Lb TDC was performed by PCR using pET24a- tdc as a template and Phanta HS Super-Fidelity DNA Polymerase

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This means slower results, inconsistent week-to-week responses, and the frustrating sense that the protocol is not working when the real problem is preparation

Biol Chem 390, 191214.CrossRefGoogle ScholarPubMed Weschawalit, S, Thongthip, S, Phutrakool, P, et al