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These can include gastrointestinal issues, skin rashes, or allergic reactions in rare cases

Front Neural Circuits 7:116

While under anesthesia with a mixture of ketamine (120 mg/kg i.p.) and xylazine (20 mg/kg i.p.), the animal body temperature was maintained at 38C, using an electrical heated platform, and the pupils of the mice were dilated using 1% tropicamide and 2.5% phenylephrine hydrochloride applied on the corneal surface

Several neurotransmitter systems, brain-derived neurotrophic factor (BDNF), glial cells, inflammation, and neuroendocrine systems have all been implicated in the development of depression