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C57BL/6 mice were administered 4 mg of metformin twice daily (equivalent to our human experimental dose of 1000 mg twice daily) (Nair and Jacob, 2016), starting from 3 days before vaccination until either D3 (7-day course) or D10 (extended course) after vaccination, and serum was collected at D21 post-vaccination for antibody measurement (Fig

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NAFLD is associated with increased TCA flux in the liver (Rauckhorst et al., 2017, Satapati et al., 2012, Sunny et al., 2011), raising the question of whether NAFLD progression is accelerated by ER stress arising from this increased flux, and whether diminished hepatic TCA flux in mice lacking MPC activity protects the liver at least in part by alleviating or preventing ER stress

Treatment of the goat Fab fragment (0.5 mg/mL) in phosphate buffer (20 mM, pH 8.2) with dithiothreitol (0.56 mM, 40 equiv.) led to chemoselective cleavage of the interchain disulfide (Fig