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Tissue samples were cut into thin pieces and digested for 5 min with 20 mM proteinase K in a 10 mM Tris-buffer (pH 7.5), followed by incubation with 100% methanol for 30 min

26 Therefore, we suggest that hypertensive individuals with the 718C variant of the GPX4 gene are susceptible to CS because of the increased concentration or activity of the enzyme promoting proinflammatory processes and increasing the brain injury

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