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A mass spectrometry-based proteomic analysis of oxidative stress found that the electrophilic double bond and nucleophilic amino acid residues on proteins enable 4-HNE to form Michael adducts with lysine (Lys) [40], histidine (His) [41], and cysteine (Cys) [42] residues to increase the molecular masses of these amino acids by 156 Da (the molecular mass of HNE), or form Schiff-base adducts with its aldehyde group and Lys to an increase in the mass by 138 Da (Schiff-base formation with a net loss of water) [43, 44]

Even with the right diet, the elderly can experience difficulty extracting the right nutrients from their foods, and this can lead to a vitamin deficiency

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