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High-performance liquid chromatography (HPLC) analysis of chemical components in cabbage extracts Vitamin C analysis: Samples were sonicated for extraction with 50% acetonitrile, filtered through a 0.2-m polyvinylidene fluoride (PVDF) membrane filter, and used as the test solution

Sica, A., Saccani, A., Bottazzi, B., Bernasconi, S., Allavena, P., Gaetano, B., LaRossa, G., Scotton, C., Balkwill F., Mantovani, A

After washing, the slices were fixed with 4% PFA in PBS for 1 h at room temperature, and the fixation reaction was quenched with 100 mM NH 4 Cl in PBS for 30 min

HUBERMAN: So when people are combining different things in cocktail, it's not necessarily a bad thing, but you want to make sure that you're working with someone who's very familiar with peptides