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HEK293T cells stably expressing endogenous BRD4HiBiT were added at a concentration of 20,000 cells in 18 l of medium and the plate was mixed on an orbital shaker for 15 s

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On the other hand, we have not yet elucidated the potential impact of oxidation or methylation of the ET thione S to create sulfonylated ET, or S -methylated ET, respectively 67 , or insertion of an oxygen atom to the C5-position to create 5-oxo-ET 9 , or Se substitution of the S atom in ET in selenoneine 68,69 , on EgtUC ligand binding affinity