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RNA isolation and RT-qPCR analysis Inflammatory gene expression analysis was performed using total RNA extracted from cultured keratinocytes treated with SHIN1 and 18-h stimulated with IL-22, IFN- and IL-17 cytokines, as well as from both healthy and psoriasis skin explants

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Primary motor cortices of 90 days old (P90) prpTDP-43 A315T and healthy wildtype (WT) control mice were dissected out and metabolites were isolated by methanol extraction method followed by high performance mass spectrometry profiling of metabolites (Fig