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The EZ DNA Methylation-Gold Kit (Zymo Research Corp., Cat# D5005) was used for bisulfite conversion of DNA and amplification by PCR, and the Pico Methyl-Seq Library Prep Kit (Cat# D5455, D5456) was used for post-bisulfite library preparation and genome-wide bisulfite sequencing

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Transcriptome analysis of primary hBECs exposed to urban PM saw reduced expression of IL6 , a gene encoding a pro-inflammatory cytokine, and increased expression of G6PD , an antioxidant pathway gene, with vitamin D treatment [17]

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