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Histochemical and immunohistochemical staining Tissue cryosections were fixed for 15 min, incubated with SA--gal staining at 37 C overnight in the dark according to the manufacturers instructions (Solarbio, Beijing, China)

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Alpha lipoic acid (LA) Alpha lipoic acid (LA) is part of the multienzyme complex of pyruvate dehydrogenase, alpha-ketoglutarate and branched alpha-keto acids [58, 59]