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Additionally, the free and bound antibodies for infliximab, also known as total ADA, were measured using drug tolerant method

Furthermore, given that the liver is also the main site of AGE metabolism (92), in advanced liver disease, further accumulation of AGEs and activation of intrahepatic cells to produce cytokines would trigger more damaging effects on the liver due to a reduction in hepatic effective liver volume/mass (91,93)

Captured anti-PEG antibodies were colorimetrically detected using a rabbit anti-human IgG and IgM antibody conjugated to HRP (Jackson ImmunoResearch, #309-035-107) and TMB substrate

Assay: GSH/GSSG in mice cells