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Adding more water after the initial reconstitution is acceptable as long as you maintain sterile technique (alcohol swab, clean syringe)

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Cells were maintained in 5% CO 2 at 37 C and grown in DMEM medium containing 10% Fetal Bovine Serum (FBS), 2 mM L-glutamine, penicillin (50 U/mL) and streptomycin (100 mg/mL) (All medium and supplements were obtained from GIBCO)